Cloning Troll

This is a fast and hassle-free PCR primer generation BioTroll tool for cloning purposes.

1: Provide sequence names / ID's as well the vector MCS and the insert DNA sequence in the 1.st reading frame.

2: Select how the insert 3' end should be modified if e.g. a STOP codon or 3' tag is required.

3: Choose the insert 5' and 3' restriction sites.

4: Hit the Submit button.

5: Enjoy saved time. You are very welcome!

Select vector from list:

Or input custom vector information.

Vector name :

Paste vector MCS in 1st. reading frame:

Gene name / ID:

Paste gene CDS:

Add a STOP codon at the insert 3' end?

Synchronize the insert's 3' end codons with the vector MCS reading frame?

Select 5' Restriction Enzyme:
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Select 3' Restriction Enzyme:

Thank You for using BioTroll tools

For comments, suggestions and criticism: support@biotroll.com

BioTroll Tools by Dr. Peter Klein and Dr. Meenakumari Muthuramalingam

Disclaimer: Cloning Troll 2.2 was thoroughly tested for correct functionality.

However, check the output for correctness.