This is a fast and hassle-free PCR primer generation BioTroll tool for cloning purposes.
1: Provide sequence names / ID's as well the vector MCS and the insert DNA sequence in the 1.st reading frame.
2: Select how the insert 3' end should be modified if e.g. a STOP codon or 3' tag is required.
3: Choose the insert 5' and 3' restriction sites.
4: Hit the Submit button.
5: Enjoy saved time. You are very welcome!
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